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Many hydroxylated flavones are reported to have antibacterial, anticancer, or antiviral activities. In this study, eleven hydroxylated flavones including 3,3′,4′,5,7。

FeFAB cells (a kind gift from Dr Martin Löchelt, Germany) are modified CRFK cells that are used for FFV infection experiments. FeFAB cells express β-galactosidasefefab under the control of the FFV LTR, which is trans。

The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernatants 48 h later。

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